St John's Laboratory 是一家成立于2013年的英國生物科技公司，總部位于倫敦，專注于抗體試劑、試劑盒及相關(guān)生物試劑的研發(fā)與生產(chǎn)。公司以技術(shù)創(chuàng)新和數(shù)據(jù)驅(qū)動為核心發(fā)展理念，致力于為全球科研、醫(yī)療及工業(yè)客戶提供高精度、高穩(wěn)定性的實驗工具。
作為早期推動抗體驗證計劃（Antibody Validation Project, AVP）的企業(yè)之一，St John's Laboratory通過建立去中心化的質(zhì)量標(biāo)準(zhǔn)體系，與全球科研用戶共同制定驗證標(biāo)準(zhǔn)，顯著提升了產(chǎn)品的透明度和可靠性。其AVP項目不僅獲得《自然》雜志的認(rèn)可，還通過用戶共享驗證數(shù)據(jù)，形成全球科研社區(qū)的知識共享網(wǎng)絡(luò)。
公司堅持“少而精"的產(chǎn)品策略，早期聚焦抗體試劑單一品類，嚴(yán)格把控質(zhì)量，逐步擴(kuò)展至免疫組化（IHC）和體外診斷（IVD）領(lǐng)域。截至2024年，其產(chǎn)品線已覆蓋科研用抗體、ELISA試劑盒、蛋白質(zhì)分析工具等，廣泛應(yīng)用于癌癥研究、神經(jīng)科學(xué)、傳染病檢測等方向。
St John's Laboratory注重用戶體驗，設(shè)計以簡潔高效為原則，提供精準(zhǔn)搜索功能與在線客服支持，同時采用黑色主色調(diào)保護(hù)用戶視力。此外，公司通過原生內(nèi)容SEO策略積累了大量抗體驗證數(shù)據(jù)，提升產(chǎn)品在搜索引擎的可見性。
應(yīng)用：ELISA
反應(yīng)性：所有
注意：僅供科學(xué)教育研究使用（RUO）。嚴(yán)禁用于診斷或其他醫(yī)療用途。
靈敏度：1.88納克/毫升
檢測限：3.13~200納克/毫升
簡短描述：本15d-PGJ2競爭性ELISA是一種體外酶聯(lián)免疫吸附試驗，用于檢測所有血清、血漿及其他生物流體中的樣本。
背景：本ELISA試劑盒采用競爭性酶聯(lián)免疫吸附試驗（ELISA）原理。本試劑盒提供的微量酶標(biāo)板已預(yù)先包被15d-PGJ2。反應(yīng)過程中，樣本或標(biāo)準(zhǔn)品中的15d-PGJ2與固相載體上固定量的15d-PGJ2競爭結(jié)合針對15d-PGJ2的生物素標(biāo)記檢測抗體的結(jié)合位點。過量的結(jié)合物及未結(jié)合的樣本或標(biāo)準(zhǔn)品從酶標(biāo)板上洗去，隨后向每個微孔中加入抗生物素蛋白-辣根過氧化物酶（HRP）偶聯(lián)物并孵育。隨后向每個孔中加入TMB底物溶液。通過加入終止液終止酶-底物反應(yīng)，并在450 nm±2 nm波長下通過分光光度法測定顏色變化。通過與標(biāo)準(zhǔn)曲線比較樣品的光密度值（OD），即可測定樣品中15d-PGJ2的濃度。
宿主細(xì)胞：CHO細(xì)胞
注：僅限進(jìn)一步研究使用（RUO）。嚴(yán)禁用于診斷或治療用途。
簡短描述：重組人4-1BB（CD137）蛋白由CHO細(xì)胞開發(fā)，適用于研究應(yīng)用。
組織特異性 ：在活化的T細(xì)胞表面表達(dá)。
功能 ：TNFSF9/4-1BBL的受體。傳遞一種信號，可增強(qiáng)CD8(+) T細(xì)胞的存活能力、細(xì)胞毒性和線粒體活性，從而促進(jìn)抗病毒及抗腫瘤免疫（可能）。
蛋白名稱 ：腫瘤壞死因子受體超家族成員9 、4-1bb配體受體 、Cdw137 、T細(xì)胞抗原4-1bb同源物 、T細(xì)胞抗原Ila 、CD抗原Cd137
細(xì)胞定位 ：細(xì)胞膜
蛋白胨是一種噴霧干燥粉末，通過動物組織的可控酶解制成。酶包括胃蛋白酶、木瓜蛋白酶和胰酶（后者含胰蛋白酶）。
胃蛋白酶會在含有苯丙氨酸或亮氨酸鍵的任意位置切割肽鏈。
木瓜蛋白酶會在鄰近精氨酸、賴氨酸、苯丙氨酸和甘氨酸的位置切割肽鏈；胰酶則在精氨酸、賴氨酸、酪氨酸、色氨酸、苯丙氨酸和亮氨酸鍵處發(fā)揮作用。
通過水解組織可制得稻草色的蛋白胨，其營養(yǎng)豐富且易溶于水。蛋白胨含有肽類、游離氨基酸和生長因子的混合物。
由于氯化鈉濃度較低，F(xiàn)ormedium™蛋白胨非常適合酵母細(xì)胞培養(yǎng)。
室溫下干燥儲存。
組織特異性 ：在骨中高度表達(dá)。在關(guān)節(jié)軟骨和軟骨細(xì)胞中大量表達(dá)，且在骨關(guān)節(jié)炎（OA）中表達(dá)升高。在關(guān)節(jié)軟骨OA病變的軟骨細(xì)胞中與ASPN共定位。
翻譯后修飾 ：轉(zhuǎn)化生長因子β1前體蛋白：該前體蛋白在高爾基體內(nèi)被弗林蛋白酶（FURIN）切割，形成轉(zhuǎn)化生長因子β1（TGF-β-1）和潛伏相關(guān)肽（LAP）鏈，二者通過非共價鍵連接，使TGF-β-1處于失活狀態(tài)。潛伏相關(guān)肽：經(jīng)N-糖基化修飾。去糖基化可激活轉(zhuǎn)化生長因子β1（TGF-β-1）。然而，觸發(fā)TGF-β-1去糖基化激活的機(jī)制尚不清楚。
功能 ：轉(zhuǎn)化生長因子β1前體蛋白：是潛伏相關(guān)肽（LAP）和轉(zhuǎn)化生長因子β1（TGF-β-1）鏈的前體，二者分別構(gòu)成TGF-β-1的調(diào)節(jié)亞基和活性亞基。潛伏相關(guān)肽：在TGF-β-1儲存于細(xì)胞外基質(zhì)期間，維持其活性亞基處于潛伏狀態(tài)。通過與TGF-β-1非共價結(jié)合，并與LTBP1、LRRC32/GARP和LRRC33/NRROS等“微環(huán)境分子"相互作用調(diào)控其激活（這些分子控制TGF-β-1的激活）。與LRRC33/NRROS的相互作用可能調(diào)控巨噬細(xì)胞和小膠質(zhì)細(xì)胞中TGF-β-1的激活。與LRRC32/GARP的相互作用控制活化調(diào)節(jié)性T細(xì)胞（Tregs）表面TGF-β-1的激活。與整合素（ITGAV:ITGB6或ITGAV:ITGB8）結(jié)合會導(dǎo)致潛伏相關(guān)肽鏈構(gòu)象改變，進(jìn)而釋放活性TGF-β-1。
轉(zhuǎn)化生長因子β1：一種多功能蛋白，調(diào)控多種細(xì)胞類型的生長與分化，并參與正常發(fā)育、免疫功能、小膠質(zhì)細(xì)胞功能及神經(jīng)退行性疾病應(yīng)答等多種過程。其激活為成熟形式需經(jīng)歷多個步驟：前體蛋白在高爾基體內(nèi)切割后，潛伏相關(guān)肽（LAP）和轉(zhuǎn)化生長因子β1（TGF-β-1）鏈仍通過非共價鍵連接，使TGF-β-1在儲存于細(xì)胞外基質(zhì)期間保持失活狀態(tài)。同時，LAP鏈與LTBP1、LRRC32/GARP和LRRC33/NRROS等“微環(huán)境分子"相互作用，這些分子控制TGF-β-1的激活并使其在細(xì)胞外環(huán)境中儲存時維持潛伏狀態(tài)。TGF-β-1通過整合素（ITGAV:ITGB6或ITGAV:ITGB8）從LAP上釋放：整合素與LAP結(jié)合可穩(wěn)定LAP“蝴蝶結(jié)尾"結(jié)構(gòu)的另一種構(gòu)象，導(dǎo)致LAP鏈構(gòu)象改變并釋放活性TGF-β-1。釋放LAP后激活的TGF-β-1通過與TGF-β受體（TGFBR1和TGFBR2）結(jié)合發(fā)揮作用（受體介導(dǎo)信號轉(zhuǎn)導(dǎo)）。盡管多種細(xì)胞類型均可表達(dá)TGF-β-1，但其活性僅局限于細(xì)胞微環(huán)境內(nèi)，這得益于LAP鏈和“微環(huán)境分子"對其激活的精細(xì)調(diào)控。在骨重塑中起重要作用：作為成骨細(xì)胞骨形成的強(qiáng)效刺激因子，可誘導(dǎo)定向成骨細(xì)胞的趨化、增殖和分化。其濃度依賴性可促進(jìn)輔助性T細(xì)胞17（Th17）或調(diào)節(jié)性T細(xì)胞（Treg）譜系分化：高濃度時，通過FOXP3介導(dǎo)抑制RORC并下調(diào)IL-17表達(dá)，促進(jìn)Treg細(xì)胞發(fā)育；低濃度時，與IL-6和IL-21協(xié)同作用，誘導(dǎo)IL-17和IL-23受體表達(dá)，促進(jìn)向Th17細(xì)胞分化。通過調(diào)控性膜內(nèi)蛋白水解（RIP）激活CREB3L1，刺激膠原蛋白持續(xù)產(chǎn)生。通過誘導(dǎo)SMAD2/3磷酸化并隨后轉(zhuǎn)位至細(xì)胞核，介導(dǎo)SMAD2/3激活。通過促進(jìn)IPO7介導(dǎo)的磷酸化SMAD2核轉(zhuǎn)位及后續(xù)靶基因轉(zhuǎn)錄，正向調(diào)控牙乳頭細(xì)胞中的成牙本質(zhì)細(xì)胞分化。可在多種細(xì)胞類型中誘導(dǎo)上皮間質(zhì)轉(zhuǎn)化（EMT）和細(xì)胞遷移。
蛋白質(zhì)名稱 ：轉(zhuǎn)化生長因子β1前體蛋白（可切割為潛伏相關(guān)肽（LAP）-轉(zhuǎn)化生長因子β1（TGF-β-1））
技術(shù)性：專注于抗體研發(fā)與驗證技術(shù)，參與制定行業(yè)質(zhì)量標(biāo)準(zhǔn)。
數(shù)據(jù)驅(qū)動品控：通過AVP項目積累上千份用戶驗證數(shù)據(jù)，確保產(chǎn)品可靠性。
垂直領(lǐng)域深耕：聚焦免疫組化與體外診斷，提供細(xì)分場景解決方案。
全球化服務(wù)網(wǎng)絡(luò)：支持多語言、多地區(qū)配送，覆蓋90多個國家客戶。
客戶協(xié)同創(chuàng)新：與科研機(jī)構(gòu)合作開發(fā)定制化產(chǎn)品，加速技術(shù)轉(zhuǎn)化。
科研機(jī)構(gòu)：大學(xué)實驗室、研究所（如癌癥研究中心、神經(jīng)科學(xué)團(tuán)隊）。
醫(yī)療領(lǐng)域：醫(yī)院病理科、第三方診斷實驗室。
工業(yè)客戶：生物制藥企業(yè)、體外診斷試劑開發(fā)商。
教育機(jī)構(gòu)：生命科學(xué)教學(xué)實驗室、技術(shù)培訓(xùn)中心。
Q1：產(chǎn)品質(zhì)量如何保障？
A：所有產(chǎn)品均通過抗體驗證計劃（AVP），提供第三方用戶驗證數(shù)據(jù)；生產(chǎn)流程符合ISO 13485標(biāo)準(zhǔn)。
Q2：是否支持定制化開發(fā)？
A：可提供抗體標(biāo)記、試劑盒優(yōu)化等服務(wù)，需聯(lián)系技術(shù)團(tuán)隊評估需求。
Q3：運輸時效與冷鏈管理如何？
A：全球主要地區(qū)3-5工作日送達(dá)，全程溫控物流，提供干冰包裝。
Q4：文獻(xiàn)引用是否有支持？
A：提供產(chǎn)品相關(guān)參考文獻(xiàn)列表，并可協(xié)助檢索特定研究場景的應(yīng)用案例。
貨號 | 品名 | 規(guī)格 | 品牌 |
STJ11101522-100uL | Anti-pan-Kla antibody (STJ11101522) | 100uL | St John's Laboratory |
STJE0005616 | Rat C3 (Complement C3) Sandwich ELISA Kit (STJE0005616) | 96T | St John's Laboratory |
STJ11101522-20uL | Anti-pan-Kla antibody (STJ11101522) | 20uL | St John's Laboratory |
STJ195089-100ug | Anti-TOP6BL antibody (STJ195089) | 100ug | St John's Laboratory |
STJ195019-20ug | Anti-ZBED6 antibody (STJ195019) | 20ug | St John's Laboratory |
STJ193142-20ug | Anti-OGG1 antibody (STJ193142) | 20ug | St John's Laboratory |
STJ112074-200uL(2x100uL) | Anti-UPK3A antibody (19-207) (STJ112074) | 200uL(2x100uL) | St John's Laboratory |
STJ72990-100ug | Anti-Pycard (rodent) Antibody | 100ug | St John's Laboratory |
STJ194899-100ug | Anti-C8orf82 antibody | 100 ug | St John's Laboratory |
STJ195089-50ug | Anti-TOP6BL antibody (STJ195089) | 50ug | St John's Laboratory |
STJ194662-20ug | Anti-TARM1 antibody (36-86 aa) (STJ194662) | 20ug | St John's Laboratory |
STJ194640-20ug | Anti-SLC25A44 antibody (237-287 aa) (STJ194640) | 20ug | St John's Laboratory |
STJ194519-50ug | Anti-SWSAP1 antibody | 50ug | St John's Laboratory |
STJ194519-20ug | Anti-SWSAP1 antibody | 20ug | St John's Laboratory |
STJ195930-20ug | Anti-ZSWIM7 antibody | 20ug | St John's Laboratory |
STJ195930-50ug | Anti-ZSWIM7 antibody | 50ug | St John's Laboratory |
STJ194378-200ug | Anti-TSACC antibody (57-107 aa) (STJ194378) | 200ug | St John's Laboratory |
STJ194295-200ug | Anti-KCNT2 antibody (689-739 aa) (STJ194295) | 200ug | St John's Laboratory |
STJ197760-20ug | Anti-SLC26A1 antibody (502-552 aa) (STJ197760) | 20ug | St John's Laboratory |
STJ193511-100ug | Anti-ERFE antibody (102-152 aa) (STJ193511) | 100ug | St John's Laboratory |
STJ192833-100ug | Anti-OR52L1 antibody (190-270 aa) (STJ192833) | 100ug | St John's Laboratory |
STJ502567-100ug | Anti-Phospho-KIRREL-p737/738 antibody (STJ502567) | 100 ug | St John's Laboratory |
STJ71686 | Anti-TROP2 Antibody | 100ug | St John's Laboratory |
STJ91023-50ug | Anti-Phospho-TH-Ser19 antibody (10-59 aa) (STJ91023) | 50ug | St John's Laboratory |
STJ119906-100ul | Anti-PDE3A antibody (380-660) (STJ119906) | 100ul | St John's Laboratory |
STJ11105344-20uL | Anti-GSDMD antibody (1-100, N-Term) (STJ11105344) | 20uL | St John's Laboratory |
STJE0004160 | Monkey VWF (Von Willebrand Factor) Sandwich ELISA Kit | 96T | St John's Laboratory |
STJE0003503 | Human VWF (von Willebrand factor) Sandwich ELISA Kit | 96T | St John's Laboratory |
STJ500339 | Anti-CDH17 antibody (300-350) | 100 ug | St John's Laboratory |
09-8922-100 | Carbonate bi-carbonate | 100 tablets | St John's Laboratory |
STJE0010009-96T | Mouse INHbE (Inhibin Beta E) Sandwich ELISA Kit (STJE0010009) | 96T | St John's Laboratory |
STJ195130-100ug | Anti-TMEM242 antibody (53-103 aa) (STJ195130) | 100ug | St John's Laboratory |
STJ195178-200ug | Anti-TMEM198 antibody (273-323 aa) (STJ195178) | 200ug | St John's Laboratory |
STJ195178-20ug | Anti-TMEM198 antibody (273-323 aa) (STJ195178) | 20ug | St John's Laboratory |
STJ195296-100ug | Anti-TMEM88B antibody | 100ug | St John's Laboratory |
STJ195296-200ug | Anti-TMEM88B antibody (STJ195296) | 200ug | St John's Laboratory |
STJ195296-20ug | Anti-TMEM88B antibody | 20ug | St John's Laboratory |
STJE0004984 | Mouse Il2rb (Interleukin-2 receptor subunit beta) Sandwich ELISA Kit | 96T | St John's Laboratory |
STJ195296-50ug | Anti-TMEM88B antibody | 50ug | St John's Laboratory |
STJ195593-20ug | Anti-PERM1 antibody (434-484 aa) (STJ195593) | 20ug | St John's Laboratory |
STJ195701 | Anti-MTLN antibody (STJ195701) | 20ug | St John's Laboratory |
STJ195701-100ug | Anti-YB039 antibody (STJ195701) | 100ug | St John's Laboratory |
STJ195701-200ug | Anti-YB039 antibody (STJ195701) | 200ug | St John's Laboratory |
STJ195721-100ug | Anti-DNAI4 antibody (281-331 aa) (STJ195721) | 100ug | St John's Laboratory |
STJ195768-200ug | Anti-STON2 antibody (47-97 aa) (STJ195768) | 200ug | St John's Laboratory |
STJ195793-100ug | Anti-TMEM252 antibody (21-71 aa) (STJ195793) | 100ug | St John's Laboratory |
STJE0003230-96T | Human TAT (Tyrosine aminotransferase) Sandwich ELISA Kit (STJE0003230) | 96T | St John's Laboratory |
STJ195920-100ug | Anti-MFSD12 antibody (78-128 aa) (STJ195920) | 100ug | St John's Laboratory |
STJE0002568 | Human OGA (Protein O-GlcNAcase) Sandwich ELISA Kit | 96T | St John's Laboratory |
STJE0002024 | Human LPA (Apolipoprotein (a)) Sandwich ELISA Kit | 96 T | St John's Laboratory |
STJ195998-100ug | Anti-SELENOH antibody (58-108 aa) (STJ195998) | 100ug | St John's Laboratory |
STJ196333-100ug | Anti-Phospho-IRF7-Ser477 antibody (STJ196333) | 100ug | St John's Laboratory |
STJA0010492-50uL | Anti-METTL3 antibody [SM0492] (STJA0010492) | 50uL | St John's Laboratory |
STJ196333-20ug | Anti-Phospho-IRF7-Ser477 antibody (STJ196333) | 20ug | St John's Laboratory |
STJ196570-100ug | Anti-SCGB1D4 antibody (STJ196570) | 100ug | St John's Laboratory |
STJ196570-200ug | Anti-SCGB1D4 antibody (STJ196570) | 200ug | St John's Laboratory |
STJA0010492-100uL | Anti-METTL3 antibody [SM0492] (STJA0010492) | 100uL | St John's Laboratory |
STJ196570-20ug | Anti-SCGB1D4 antibody (STJ196570) | 20ug | St John's Laboratory |
STJ197447-20ug | Anti-STIP1 antibody (250-300 aa) (STJ197447) | 20ug | St John's Laboratory |
STJ197459-20ug | Anti-Phospho-H2AX-Tyr142 antibody (STJ197459) | 20ug | St John's Laboratory |
STJ197760-100ug | Anti-SLC26A1 antibody (502-552 aa) (STJ197760) | 100ug | St John's Laboratory |
STJA0008863-50ug | Anti-CWF19L2 antibody (STJA0008863) | 50ug | St John's Laboratory |
STJ197760-50ug | Anti-SLC26A1 antibody (502-552 aa) (STJ197760) | 50ug | St John's Laboratory |
STJ198363-50ug | Anti-NDUFAF6 antibody (STJ198363) | 50ug | St John's Laboratory |
STJ22058-20uL | Anti-Phospho-CDK1-T14 antibody (STJ22058) | 20uL | St John's Laboratory |
STJ22524-20uL | Anti-ADNP antibody (843-1102) (STJ22524) | 20uL | St John's Laboratory |
stj23004-100uL | Anti-CD47 antibody (292-304) | 100uL | St John's Laboratory |
STJ24191-200uL(2x100uL) | Anti-IL7 antibody (26-177) (STJ24191) | 200uL(2x100uL) | St John's Laboratory |
STJ24688-100uL | Anti-NBAS antibody (1-70) (STJ24688) | 100uL | St John's Laboratory |
STJ24688-20uL | Anti-NBAS antibody (1-70) (STJ24688) | 20uL | St John's Laboratory |
STJ25110-100uL | Anti-PRG2 antibody (100-200) (STJ25110) | 100uL | St John's Laboratory |
STJ26000-100ul | Anti-TYK2 antibody (750-850) (STJ26000) | 100ul | St John's Laboratory |
STJ26000-50ul | Anti-TYK2 antibody (750-850) (STJ26000) | 50ul | St John's Laboratory |
STJ26764-50ul | Anti-TIMM10 antibody (1-90) (STJ26764) | 50uL | St John's Laboratory |
STJ28375-100uL | Anti-COMP antibody (20-160) (STJ28375) | 100uL | St John's Laboratory |
STJ28375-200uL(2x100uL) | Anti-COMP antibody (20-160) (STJ28375) | 200uL(2x100uL) | St John's Laboratory |
STJ28375-20uL | Anti-COMP antibody (20-160) (STJ28375) | 20uL | St John's Laboratory |
STJ28773-100uL | Anti-PLA2G2D antibody (21-145) (STJ28773) | 100uL | St John's Laboratory |
STJA0003869-100uL | Anti-CD71 antibody [DF1513] | 100uL | St John's Laboratory |
STJ29153-200uL(2x100uL) | Anti-LILRB4 antibody (22-259) | 200uL(2x100uL) | St John's Laboratory |
STJA0002876-100ug | Anti-DNase I antibody [4B10] (STJA0002876) | 100ug | St John's Laboratory |
STJ29417-100uL | Anti-CD47 antibody (20-140) (STJ29417) | 100uL | St John's Laboratory |
STJ29443-20uL | Anti-SETD5 antibody (1054-1153) (STJ29443) | 20uL | St John's Laboratory |
STJ94794-20ug | Anti-OR6K2 antibody (271-320) (STJ94794) | 20ug | St John's Laboratory |
STJ95929-100ug | Anti-TBX1 antibody (311-360 aa) (STJ95929) | 100ug | St John's Laboratory |
STJ95867-100ug | Anti-TAS1R3 antibody (300-380 Internal) (STJ95867) | 100ug | St John's Laboratory |
STJ95822-50ug | Anti-STEAP1 antibody (31-80 aa) (STJ95822) | 50ug | St John's Laboratory |
STJ95822-20ug | Anti-STEAP1 antibody (31-80 aa) (STJ95822) | 20ug | St John's Laboratory |
STJ95822-100ug | Anti-STEAP1 antibody (31-80 aa) (STJ95822) | 100ug | St John's Laboratory |
STJ95149-20ug | Anti-PKP2 antibody (632-681 aa) (STJ95149) | 20ug | St John's Laboratory |
STJ94794-50ug | Anti-OR6K2 antibody (271-320) (STJ94794) | 50ug | St John's Laboratory |
STJ94134-100ug | Anti-MITF antibody (120-200 aa) (STJ94134) | 100ug | St John's Laboratory |
STJ93218-20ug | Anti-GAS6 antibody (291-340 aa) (STJ93218) | 20ug | St John's Laboratory |
STJ93385-20ug | Anti-FFAR3 antibody (11-60 aa) (STJ93385) | 20ug | St John's Laboratory |
STJ93908-50ug | Anti-LATS1/2 antibody (1010-1090) (STJ93908) | 50ug | St John's Laboratory |
STJ95929-20ug | Anti-TBX1 antibody (311-360 aa) (STJ95929) | 20ug | St John's Laboratory |
STJ98607-100ug | Anti-TPSD1 antibody (51-100 aa) (STJ98607) | 100ug | St John's Laboratory |
